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Fig. 7. MIA-induced osteoarthritic pain was markedly relieved by intrathecal injection of TAP2 by inhibiting microglia activation. (A) On day 7 post-injection of MIA (2 mg in 25 µL PBS) into the knee joint of the left hind leg, rats were randomly divided into two groups and intrathecally administered PBS or TAP2 (25 nmol in 20 µL PBS). The mechanical threshold was then examined from days 1 to 24 post-injection. Data are expressed as means±SEM (two-way ANOVA with Bonferroni post hoc test, ***p<0.001, **p<0.01, and *p<0.05 vs. MIA+PBS; n=5 per group). (B) On day 10 after TAP2 injection, L5 spinal tissues were immunostained with anti-Iba1 antibodies. Scale bar=200 µm (left), 50 µm (right). The intensity of Iba1-immunoreacitve cells in the spinal dorsal horn was measured and presented. Sham operation was performed as a control. Data are presented as means±SEM (one-way ANOVA with Tukey's post hoc test, **p<0.01 vs. PBS; n=3 per group).
Exp Neurobiol 2019;28:352~361
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