Fig. 1. Effect of maternal DEHP exposure on cerebellar granule cell apoptosis in pups. Representative photomicrographs show fluorescence staining of terminal deoxynucleotidyl transferase-mediated dUTP nick-and labeling (TUNEL)-positive cells in the internal granular layer of cerebellum in the four groups (0, 30, 300 and 750 mg/kg/d). (A) Postnatal (PN) day 7 for males and females, (B) PN 14 for males and females. Corresponding bar graph shows the TUNEL-positive cell count in the four groups.at PN 7 (C) and PN 14 (D). Scale bar=25 µm. The scale bar is the same for all images in the figure. Western blots bands represent the expression of cleaved caspase-3 and caspase 3 for male and female offspring at PN 7 (E) and PN 14 (H). Semi-quantitative measurements of cleaved caspase-3 and total caspase 3 for male and female offspring at PN 7 (F) (G) and PN 14 (I) (J). With each time point, *p<0.05 vs control, #p<0.05 vs 30 mg/kg/d contamination group (n=6).
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