Fig. 4. Effects of MEHP on cell viability of primary CGCs. (A) Representative photomicrographs show the intact morphological structure of primary cerebellar granule cell neurons. Positive staining cells (yellow arrow) are CGCs and negative staining cells (white arrow) indicate non-CGCs, Scale bar=100 µm. (B) CGCs were exposed to various concentrations of mono-(2-ethylhexyl) phthalate (MEHP; 0~1000 µM) for 12 h, 24 h and 48 h, and cell viability was measured by CCK-8 kit. Data are expressed as the mean±SD for three independent experiments. Each assay was performed in triplicate *p<0.05 vs control.
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