Fig. 5. Analyses of AQP4 polarization and expression. (A) Immunofluorescent staining of AQP4 in the hippocampus. An abnormal increase in AQP4 expression was observed in the parenchymal domains (star) of SAH mice. (B) Relative pixel intensity of AQP4 immunofluorescence. (C) Relative perivascular polarization of AQP4. (D~E) Representative bands of Western blotting and corresponding densitometry analysis for expression levels of AQP4 in the dorsal forebrain from three independent experiments. Results expressed as mean±SEM from 4 mice per group. The statistical analysis was performed by Student's t-test. *p<0.05; **p<0.01.
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