Fig. 7. Analyses of neuronal apoptosis. (A) Immunohistochemical staining for caspase-3 in the dentate gyrus of hippocampus. (B) Percentages of caspase-3-positive apoptotic neurons. (C~D) Representative bands of Western blot and corresponding densitometry analysis for expression levels of cleaved caspase-3, Bax, and Bcl-2 in the dorsal forebrain. Graphs are the summary of results from three independent experiments. Data expressed as mean±SEM from 4 (B) or 5 (D) mice per group. Statistical analysis was performed by Student's t-test. *p<0.05, ***p<0.001.
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