Fig. 7. Comparison between diffusion tractography and neuronal tracer projection. The neuronal injection site regions of interest (colored in light blue) were first registered to our native mouse brain diffusion image then used to generated diffusion tractography. Then the diffusion tractography fibers (colored in red-orange) generated from the injection ROI were registered back to ABA mouse template (obtained using ABA software development kit
http://alleninstitute.github.io/AllenSDK/connectivity.html) and was compared with neuronal tracer projection data (colored in green). All injection, projection and diffusion tractography data were overlaid on top of ABA mouse template. (A) Sagittal view of the injection, projection, diffusion tractography, ABA template overlay. (B) Coronal view of the injection, projection, diffusion tractography, ABA template overlay.
