Fig. 2. Construction of a brain atlas of adult
Octopus minor. (A) Genotyping of the animals used in atlas construction. ClustalW multiple sequence alignment of the mitochondrial cytochrome oxidase subunit I gene with known sequences of Haplotype 1 and Haplotype 6 [
14]. The 12 nucleotide polymorphisms from Kang's study [
14] are depicted as colored boxes, with the nucleotide number. Asterisks indicate nucleotides identical to those in Haplotype 1. (B) Schematic diagram of the sagittal section and established coordinates of the brain. (C) Representative images of the sagittal sections visualized by Hematoxylin and Eosin staining, shown with their coordinates. Scale bar=2 mm. (D) Representative images of delineations with their coordinates drawn on a selected sagittal section. Scale bar=2 mm. (E) Schematic diagram of the coronal section and established coordinates of the brain. (F) Representative images of the coronal sections visualized by Hematoxylin and Eosin staining, shown with their coordinates. Scale bar=2 mm. (G) Representative images of delineations with their coordinates drawn on a selected coronal section. Scale bar=2 mm. AP, anterior-posterior axis; ML, mediallateral axis; ±, coordinate direction; Asterisk, fiducial point; A, anterior; P, posterior; D, dorsal; V, ventral; M, medial; L, lateral.
