Fig. 4. High-power microscopic view of tissue stained with various stains. (A~C) A spheroid (arrow) is rarely observed in the white matter of the cerebral hemisphere (A). Spheroids are emphasized via Bielschowsky silver staining (B) and neurofilament staining (C); axonal loss can also be observed with these stains. (D) The spheroids (arrows) are occasionally observed in the cortex of the parietal lobe and the density is higher than that of spheroids in the white matter. (E~H) Macrophage shows hyperchromatic nuclei with brown-pigmented cytoplasm and round cellular contour (arrows) (E). CD68 immunohistochemistry shows a mostly negative status in pigmented cells (F). CD163 staining shows pigmented glial component with a ramification pattern. (G) Periodic acid-Schiff (PAS) also stained pigmented cells (H). (I) Reactive gliosis is observed in the white matter of the frontal lobe. (J) The white matter of the cerebellum is also involved and is accompanied by parenchymal vacuolation. The inset is a low-power view of the image in J., and the lesion is located in the subcortical area of the vermis. (K~L) p-Tau (AT8) was negative in both hippocampal formation (K) and disease affected cerebral neocortex (L). (M~N) p-TDP43 was also negative in both hippocampal formation (M) and disease affected cerebral neocortex (N). (A, D, E, I, J: Hematoxylin and eosin, B: Bielschowsky silver, C: Neurofilament, F: CD68, G: CD163, H: Periodic acid-Schiff, K, L: p-Tau (AT8) M, N: p-TDP43).
© Exp Neurobiol
