Primary antibodies used in this case for diagnosis
AntibodiesDilutionCompanyFindings in this case
GFAP1:300DAKO, Glostrup, Denmark+ in reactive astrocytes
NeuN1:500Millipore, Temecula, USA+ in neurons
Neurofilament (NF)1:2000DAKO, Glostrup, Denmark+ in axons and axonal spheroids
Phosphorylated NF1:10,000Millipore, Temecula, USA+ in axons and axonal spheroids
Synaptophysin1:100Novocastra, Newcastle, UK+ in gray matter
CD1631:200ABCAM, Bristol, UK+ in pigmented microglia
CD681:2000DAKO, Glostrup, Denmark+ in a few of the pigmented microglia
α-synuclein1:200ABCAM, Bristol, UKNegative in entire brain
β-amyloid1:500Covance, Dallas, USANegative in entire brain
3 repeat (3R) tau1:100Millipore, Ontario, CanadaNegative in entire brain
4 repeat (4R) tau1:1000Millipore, Ontario, CanadaNegative in entire brain
p-Tau (AT8)1:100ThermoFisher,Waltham, USANegative in entire brain
p-TDP431:1,000Cosmobio, Tokyo, JapanNegative in entire brain

GFAP, Glial fibrillary acidic protein; NeuN, Neuronal nuclei; CD, Cluster of differentiation; p-Tau, phospho-Tau; p-TDP43, phosphorylated TAR DNA binding protein.

Exp Neurobiol 2019;28:119~129 https://doi.org/10.5607/en.2019.28.1.119
© Exp Neurobiol