Antibodies | Dilution | Company | Findings in this case |
---|---|---|---|
GFAP | 1:300 | DAKO, Glostrup, Denmark | + in reactive astrocytes |
NeuN | 1:500 | Millipore, Temecula, USA | + in neurons |
Neurofilament (NF) | 1:2000 | DAKO, Glostrup, Denmark | + in axons and axonal spheroids |
Phosphorylated NF | 1:10,000 | Millipore, Temecula, USA | + in axons and axonal spheroids |
Synaptophysin | 1:100 | Novocastra, Newcastle, UK | + in gray matter |
CD163 | 1:200 | ABCAM, Bristol, UK | + in pigmented microglia |
CD68 | 1:2000 | DAKO, Glostrup, Denmark | + in a few of the pigmented microglia |
α-synuclein | 1:200 | ABCAM, Bristol, UK | Negative in entire brain |
β-amyloid | 1:500 | Covance, Dallas, USA | Negative in entire brain |
3 repeat (3R) tau | 1:100 | Millipore, Ontario, Canada | Negative in entire brain |
4 repeat (4R) tau | 1:1000 | Millipore, Ontario, Canada | Negative in entire brain |
p-Tau (AT8) | 1:100 | ThermoFisher,Waltham, USA | Negative in entire brain |
p-TDP43 | 1:1,000 | Cosmobio, Tokyo, Japan | Negative in entire brain |
GFAP, Glial fibrillary acidic protein; NeuN, Neuronal nuclei; CD, Cluster of differentiation; p-Tau, phospho-Tau; p-TDP43, phosphorylated TAR DNA binding protein.