Fig. 6. DA increases GABA production via MAO-B in primary hypothalamic astrocytes. (A) Timeline of treatment of primary hypothalamic astrocytes with DA for 24 h or 30 min followed by immunocytochemistry. (B) Representative fluorescence immunocytochemical images of cells treated with 100 µM DA with different durations. Cells were treated with 100 µM GABA as a positive control. (C) Fluorescence intensity of GABA labeling in GFAP-positive primary hypothalamic astrocytes. All data are shown as mean±SEM (0 µM, n=50 cells; 100 µM for 24 h, n=43 cells; 100 µM for 24 h with selegiline, n=40 cells; 100 µM for 30 min, n=47 cells; and GABA, n=39 cells) and were analyzed using Tukey's multiple comparison test. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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