Fig. 2. D2 heterozygous pups exhibited increased HPA axis response to early-life stress. (A) Experimental design for treatment with early-life stress and time points for obtaining samples. Red arrows, time points for sacrifice. (B) Corticosterone levels of WT and D2+/− pups raised normally or after exposure to ELS. ELS-exposed groups and their control pups were sacrificed on postnatal day 14. n=4~5 animals for each group, each with duplicate. (ELS effect, F(1, 33)=7.421, p=0.0102; genotype effect, F(1, 33)=0.1043, p=0.7488; genotype x ELS interaction, F(1, 33)=2.466, p=0.1259). (C) Corticosterone levels of WT and D2+/− mice raised normally or after exposure to ELS, and sacrificed on postnatal day 70. To induce a stress response, the mice were exposed to 60-min restraint (rst-60) and then sacrificed. n=4~7 animals for each group, each with duplicate. Data are presented as mean±SEM. * and ** denote differences between the indicated groups at p<0.05 and p<0.01, respectively (One-way, two-way ANOVA and Holm-Sidak post hoc test).
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