Fig. 1. CCI induced hyperalgesia and P2X7R upregulation in the spinal dorsal horn. (A) CCI induced mechanical hyperalgesia. The mechanical paw withdrawal threshold on day 7 following CCI surgery was significantly lower than that in the sham group (n=6, mean±SEM, ***p<0.001, ANOVA). (B) CCI induced thermal hyperalgesia. The thermal paw withdrawal latency on day 7 following CCI surgery was significantly lower than that in the sham group (n=6, mean±SEM, p<0.01, ANOVA). (C) Activation of P2X7R/ERK signaling in the spinal dorsal horn after CCI. (D) Upregulation of P2X7R in the spinal dorsal horn after CCI. Western blotting showed that the relative expression of P2X7R was significantly higher in the CCI group than in the sham group (n=4, mean±SEM, *p<0.05, ANOVA). (E) Upregulation of PERK in the spinal dorsal horn after CCI. Western blotting showed that the relative expression of PERK was significantly higher in the CCI group than in the sham group (n=4, mean±SEM, *p<0.05, ANOVA). CCI, chronic constriction injury; Contra, contralateral to CCI; NC, negative control. Scale bar, 100 µm.
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