Fig. 1. Efficient clearance of FxClear (Fix-Clear) technique processed tissue. (A) Comparison of procedures and processing times between FxClear and other clearing methods. (B) Transparency of brain tissue during the FxClear process (n=6, each time point) and comparison with ACT 1 h (n=5) and FASTClear 12 h (n=5). (C) Representative images of cleared brain slice before and after refractive index (RI) matching (top) (square units; x: 5 mm, y: 5 mm). Comparison of tissue size change during the tissue clearing process with FxClear, ACT and FASTClear at indicated times of process (bottom) (n=5; *p<0.05, ***p<0.001).
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