Fig. 3. FxClear technique maintains fluorescence protein signals. (A) Protein loss in a 1 mm-thick brain slice during the FxClear process until 80 h (n=3, each time point). (B) The integrated eYFP cellular fluorescence intensity of FxClear 3 h and ACT 1 h cleared 1 mm Thy1-eYFP mouse brain slices (n=6 slices). Scale bar, 25 µm. (C) Comparison of collagen type IV antibody labeling depths and z-plot profiles (left) in PFA-only, FxClear 3 h and ACT 1 h 1 mm-thick brain slices (n=5 slices). Scale bar, 50 µm.
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