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Fig. 5. Thick-brain slices and peripheral organs tissue clearing using FxClear. (A) Thick mouse brain slices (2~3 mm) processed with 12 h of FxClear. Scale bar, 1 cm. (B) Comparison of transparency after FxClear with different tissue thicknesses (n=4; **p < 0.01; ns, non-specific). (C) FxClear-processed 2-mm-thick brain slice stained with anti-collagen type IV (red), anti-GFAP (green) antibodies and DAPI (blue). (z=1.48 mm imaging stack depth left). Large-magnification image shows the interaction of astrocytes with vasculature at cellular resolution (right). Scale bar, 200 µm (left), 50 µm (right). (D) FxClear-processed 3-mm-thick cerebellum region stained with antibodies against GFAP (red), Calbindin (green) and collagen type IV (blue). (z=0.87 mm). Scale bar, 100 µm. (E) Representative images of organs before and after FxClear (rat embryo, mouse whole brain, liver, kidney). After FxClear processing, organs were treated with CIBIC-mount for 1 day to adjust the refractive indices (square units; x: 5 mm, y: 5 mm). (F) FxClear-processed embryonic day 12.5 Tau-GFP mouse was stained with anti-GFP (green) and anti-Laminin (red) (z=1.49 mm). Scale bar, 1 mm.
Exp Neurobiol 2019;28:436~445
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