Fig. 1. Neural progenitor cells (NPCs) overexpressing arginine decarboxylase (ADC) genes (ADC-NPCs) attenuate cell death and cell cycle exit following ischemic stress. (A) Western blot of NPCs and (B) quantification graph indicating that the expression level of ADC in the ADC-NPCs increased under both normal and oxygen-glucose deprivation (OGD) conditions (n=3~4 per condition). (C) Immunocytochemical staining of ADC and agmatine, and Hoechst 33342 (Hxt33342)-propidium iodide (PI) staining in NPCs under both normal and OGD conditions. (D) Quantification graph of Hxt33342-PI staining in NPCs under both normal and OGD conditions. Overexpression in ADC-NPCs increases the number of PI-positive dead cells. (E) Fluorescence-activated cell sorting (FACS) analysis of PI-positive NPCs shows the cell cycle states under both normal (a) and ischemic conditions (b) (n=7~8 per condition). The error bars represent the mean±SEM. ADC, human arginine decarboxylase; NC, normal conditions; OGD 2hr, 2-h oxygen-glucose deprivation injury. White arrows indicate positive cells.
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