Fig. 2. Neural progenitor cells (NPCs) overexpressing arginine decarboxylase (ADC) genes (ADC-NPCs) promote neural differentiation following ischemic stress. (A) Western blot of NPCs and (B) quantification graph showing the increase in the expression levels of neural marker DCX and oligodendroglial marker Olig2, and the decrease in the expression level of astroglial marker GFAP in ADC-NPCs following oxygen-glucose deprivation (OGD) (n=5~7 per condition). (C~E) Immunocytochemical staining of the NPCs and quantification graphs showing neural differentiation indicated by labelling with: (C) DCX (green) and nestin (red); (D) S100β (green) and GFAP (red); and (E) MBP (green) and Olig2 (red) antibodies. We used 4′,6-diamidino-2-phenylindole (DAPI) as a nucleus for counterstaining (blue). The error bars represent the mean±SEM. ADC, human arginine decarboxylase; NC, normal conditions; OGD 2hr, 2-h oxygen-glucose deprivation injury. White arrows indicate positive cells.
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