Fig. 4. Transforming growth factor beta (TGFβ) expression in activated CD68-positive cells in the chronic stage. (A) Paraffin-embedded brain sections were immunostained with CD68 (red) for phagocytic cells and TGFβ (green) for anti-inflammatory cytokines and counterstained with DAPI (blue) for cell nuclei at one month after reperfusion. Both phagocytic and anti-inflammatory cytokine-expressing cells are shown in the inset images. 1, infarct core; 2, peri-infarct core; 3, contralateral hemisphere. Representative images were obtained from animal R330. Scale bar represents 10 μm. (B~D) Quantification of CD68- and TGFβ-positive cells. The number of CD68-specific (B), TGFβ-specific (C), and CD68+/TGFβ+-coexpressing (D) cells was quantified using Image J cell counter. (E) The ratio of CD68+/TGFβ+ cells over total TGFβ+ cells is shown. Data are presented as mean±SEM. Each line chart symbol represents 50 regions of interest, with 2 animals in each group. (F) Linear regression analysis of the intensities of CD68- and TGFβ-positive cells in the ipsilateral hemisphere from the subacute and chronic groups.
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