Primer sequences for RT-PCR
| Sense primer (5′→3′) | Antisense primer (5′→3′) | Product size (bp) | |
|---|---|---|---|
| Exon 1–4 | 1:TCCAGCCTCTCTGCCTACTC | 5:CGGGGTTTTATGGATGATTTT | 424 bp (intact) |
| Exon 2–3 | 2:CGAGTTAAAGTTGGACGTTCTGG | 3:AGGTTCGGAGCCCAATTTTGT | 115 bp |
| Exon 3–4 | 4:AGTGGCCATATCCATGAACTG | 5:CGGGGTTTTATGGATGATTTT | 194 bp |
| Exon 22–23 | 6:CAGTAAAGAGTCTCACGCTACAG | 7:TGTTCCAGGGATGTCACCAGA | 110 bp |