Primer sequences for RT-PCR
| Gene | Primer sequence | |
|---|---|---|
| IL-1β (Interleukin-1 beta) | Forward | 5′-CTG GTG TGT GAC GTT CCC ATT A-3′ |
| Reverse | 5′-CCG ACA GCA CGA GGC TTT-3′ | |
| TLR2 (Toll-like receptor 2) | Forward | 5′-TGC TTT CCT GCT GGA GAT TT-3′ |
| Reverse | 5′-TGT AAC GCA ACA GCT TCA GG-3′ | |
| TLR4 (Toll-like receptor 4) | Forward | 5′-ACC TGG CTG GTT TAC ACG TC-3′ |
| Reverse | 5′-CTG CCA GAG ACA TTG CAG AA-3′ | |
| SRA (Scavenger receptor A) | Forward | 5′-GAC GCT TCC AGA ATT TCA GC-3′ |
| Reverse | 5′-ATG TCC TCC TGT TGC TTT GC-3′ | |
| TNF-α (Tumor necrosis factor-α) | Forward | 5′-TTC TGT CTA CTG AAC TTC GGG-3′ |
| Reverse | 5′-GTA TGA GAT AGC AAA TCG GCT-3′ | |
| CD45 (Protein tyrosine phosphatase, receptor type, C) | Forward | 5′-CAG AGC ATT CCA CGG GTA TT-3′ |
| Reverse | 5′-GGA CCC TGC ATC TCC ATT TA-3′ | |
| CD11b (Integrin alpha M) | Forward | 5′-CAG ATC AAC AAT GTG ACC GTA TGG G-3′ |
| Reverse | 5′-CAT CAT GTC CTT GTA CTG CCG CTT G-3′ | |
| IκB-α (Nuclear factor kappa light polypeptide gene enhancer in B-cells inhibitor-α) | Forward | 5′-GAA GCC GCT GAC CAT GGA A-3′ |
| Reverse | 5′-GAT CAC AGC CAA GTG GAG TGG A-3′ | |
| GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) | Forward | 5′-ATG AAT ACG GCT ACA GCA-3′ |
| Reverse | 5′-GCC CCT CCT GTT ATT ATG G-3′ |