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Fig. 3. Graphs illustrating the effect of i.t. administration of the NOS-II inhibitor, L-NIL on PKC- and PKA-dependent GluN1 phosphorylation in the lumbar spinal cord dorsal horn of CCI rats. (A and B) Results of Western blot analysis showed that the CCI-induced increase in PKC-dependent GluN1 phosphorylation (pGluN1) at the Ser896 site (A) and PKA-dependent pGluN1 at the Ser897 site in the spinal cord was significantly reduced by i.t. administration of L-NIL (60 nmol). The drug or vehicle was administrated twice a day from days 0 to 5 post-surgery. The spinal cord dorsal horn was sampled at 5 days post-surgery. n=6 rats/group. *p<0.05 vs. Sham; #p<0.05 vs. vehicle-treated group. A and B, one-way ANOVA followed by a Newman-Keuls multiple comparison test for post-hoc analysis.
Exp Neurobiol 2019;28:516~528
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