Fig. 6. Graphs illustrating the effect of DHEAS on the PKC- and PKA-dependent GluN1 phosphorylation originally inhibited by i.t. administration of L-NIL in CCI rats. (A and B) Results of Western blot analysis showing that co-administration of dehydroepiandrosterone sulfate (DHEAS; 10 nmol) with L-NIL (60 nmol) restored the CCI-induced increase in PKC-dependent GluN1 phosphorylation (pGluN1) at the Ser896 site (A) and PKA-dependent pGluN1 at the Ser897 site (B) in the spinal cord that were initially inhibited by administration of L-NIL (60 nmol) alone. Drugs or vehicle were administrated twice a day from days 0 to 5 post-surgery. The spinal cord dorsal horn was sampled at 5 days post-surgery. n=6 rats/group. *p<0.05, **p<0.01 vs. Sham; #p<0.05 vs. vehicle-treated group; ‡p<0.05 vs. L-NIL-treated group. A and B, one-way ANOVA followed by a Newman-Keuls multiple comparison test for post-hoc analysis.
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