Fig. 2. Downregulation of SIRT2 decreases expression of synaptic plasticity-related genes in hippocampal neurons. (A) To assess the efficiency of lenti-shSIRT2, Sirt2 and β-actin mRNA levels were quantified by qPCR (n=3). (B, C) Levels of ΔFosB and Egr1 mRNAs in mouse hippocampal primary neurons were assayed by qPCR (n=3). (D) Western blot analysis showed that expression levels of SIRT2, ΔFosB, and Egr1 were decreased by lenti-shSIRT2 infection in hippocampal primary neurons (n=3). (E) lenti-shSIRT2 infection reduced protein levels of Synaptophysin (SYP) and Synapsin1 (Syn1) (n=3). (F~I) The SIRT2-specific inhibitor, AGK2, (5 μm, 72 h) mimicked the effect of lenti-shSIRT2 infection in primary hippocampal neurons. (F, G) Levels of ΔFosB and Egr1 mRNAs in mouse hippocampal primary neurons were assayed by qPCR (n=3). (H, I) Western blot analysis showed that expression levels of ΔFosB, Egr1, and pre-synaptic molecules were decreased by AGK2 treatment in hippocampal primary neurons (n=3~4). Data are mean±s.e.m. Unpaired t tests; *p<0.05, **p<0.01.
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