Fig. 2. Transduction of Tat-AR proteins into HT-22 cells. HT-22 cell culture media were treated with Tat-AR protein at different doses (0.5~5 μM) or the AR protein for 1 h (A). The cell culture media were treated with Tat-AR protein (5 μM) or AR protein for different time periods (10~60 min) (B). Then, transduction of Tat-AR protein was measured by Western blotting and the intensity of the bands was measured by a densitometer. The localization of transduced Tat-AR protein was examined by confocal fluorescence microscopy (C). Scale bar=5 μm. Intracellular stability of transduced Tat-AR protein. HT-22 cell culture media were incubated for 36 h after transduction of Tat-AR protein for 1 h (D). Transduction of Tat-AR protein was measured by Western blotting and the intensity of the bands was measured by a densitometer. Quantitative analysis of transduced Tat-AR protein level in HT-22 cells. After HT-22 cells were treated with Tat-AR protein, transduced Tat-AR protein level determined using an ELISA kit (E). Data are repressed as mean±SEM (n=3).
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