Fig. 3. Effects of transduced Tat-AR protein on cell viability in response to oxidative stress. Effect of transduced Tat-AR protein on cell viability. HT-22 cells were pretreated with Tat-AR protein (0.5~5 μM) for 1 h and exposed to H2O2 (1 mM) for 8 h. Cell viabilities were estimated using a colorimetric assay using MTT (A). Effect of transduced Tat-AR protein on DNA damage. The cells were treated with Tat-AR (5 μM) for 1 h, and then exposed to H2O2 (1 mM) for 3 h. Then, DNA fragmentation was detected by TUNEL staining and quantitative evaluation of TUNEL positive cells confirmed by cell counting under a phase-contrast microscopy (×200 magnification) (B). Scale bar=20 μm. **p<0.01 compared with H2O2-treated cells. Data are repressed as mean±SEM (n=3).
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