Fig. 1. Inhibition of LGR5 in SH-SY5Y neuroblastoma cells. (A) Expression of LGR5 in human normal brain (N3 of ) and SH-SY5Y neuroblastoma cells. (B) Morphology of non-treated SH-SY5Y and control/LGR5 siRNA-treated SH-SY5Y, and live cell counting graphs of each groups in % of non-treated SH-SY5Y. (C) Western blotting results of SH-SY5Y treated with different concentrations of LGR5 siRNA (30, 40, 50, 80, and 160 nM). (D) Confirmation of LGR5 knockdown and downregulation of Wnt/β-catenin down-signal with 40 nM LGR5 siRNA in SH-SY5Y by western blotting.
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