Fig. 3. Effect of acute and chronic immobilization stress on proteins expression in the spinal cord. (A) JNK, (B) mTOR, (C) AMPKα1 protein phosphorylation in the spinal cord. These protein expressions were analyzed by Western blot. The number of animals in each group was 6. β-Actin (1:1000 dilution) was used as an internal loading control. Signals were quantified with the use of laser scanning densitometry and expressed as a percentage of the control. Values are mean±SEM (*p<0.05, **p<0.01, ***p<0.001; compared to Control group).
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