Inhibition of PrP^res amplification in ScN2a cells treated with 14 derivatives of BMD42-29. Effects of 14 derivatives (20 μM) (A), dose-dependent four effective derivatives (1.2~10 μM) (B) on inhibition of PrP^res amplification in ScN2a cells were assessed by western blotting. Relative units of densitometry for (A) and (B) indicate the volume intensity/mm², relative to β-actin signal in (C) and (D), respectively. Total PrP was measured in ScN2a cells treated with 14 derivatives (20 μM) (E) and dose-dependent four effective derivatives (1.2~10 μM) (F). Controls were treated with DMSO and quinacrine. Each value represents the mean±standard deviation; p<0.05. Three independent experiments were performed in duplicate, and representative immunoblots are shown. Molecular mass markers are indicated to the between two blots.