List and information of primer sets for SARS-CoV-2 detection
| Origin | Species target | Target gene | Primer name | Forward sequence (5’-3’) | Reverse sequence (5’-3’) | Final conc. | Ref. |
|---|---|---|---|---|---|---|---|
| IBS | SARS-CoV-2 | RdRP 15441-15558 | SARS-CoV-2_IBS_RdRP1 | CATGTGTGGCGGTTCACTAT | TGCATTAACATTGGCCGTGA | 0.5 μM | Current study |
| S 23114-23213 | SARS-CoV-2_IBS_S1 | CTACATGCACCAGCAACTGT | CACCTGTGCCTGTTAAACCA | ||||
| E 26259-26365 | SARS-CoV-2_IBS_E1 | TTCGGAAGAGACAGGTACGTT | CACACAATCGATGCGCAGTA | ||||
| N 28732-28849 | SARS-CoV-2_IBS_N1 | CAATGCTGCAATCGTGCTAC | GTTGCGACTACGTGATGAGG | ||||
| RdRP 15092-15193 | SARS-CoV-2_IBS_RdRP2 | AGAATAGAGCTCGCACCGTA | CTCCTCTAGTGGCGGCTATT | ||||
| RdRP 14015-14127 | SARS-CoV-2_IBS_RdRP3 | TCTGTGATGCCATGCGAAAT | ACTACCTGGCGTGGTTTGTA | ||||
| S 22340-22447 | SARS-CoV-2_IBS_S2 | GCTGGTGCTGCAGCTTATTA | AGGGTCAAGTGCACAGTCTA | ||||
| E 26259/26374 | SARS-CoV-2_IBS_E2 | TTCGGAAGAGACAGGTACGTTA | AGCAGTACGCACACAATCG | ||||
| N 28736-28855 | SARS-CoV-2_IBS_N2 | GCTGCAATCGTGCTACAACT | TGAACTGTTGCGACTACGTG | ||||
| Human IPC | RPP30 | IBS_RPP30 | CTATTAATGTGGCGATTGACCGA | TGAGGGCACTGGAAATTGTAT | 0.5 μM | Current study | |
| RPP40 | IBS_RPP40 | CTTGGCATAAAACAGGTTCAGAA | GAGATCTCTCAACGTGCTCAG | ||||
| US CDC | SARS-CoV-2 | N 28287-28358 | CDC_N1 | GACCCCAAAATCAGCGAAAT | TCTGGTTACTGCCAGTTGAATCTG | 0.05~20 μM | [11] |
| N 29164-29230 | CDC_N2 | TTACAAACATTGGCCGCAAA | GCGCGACATTCCGAAGAA | ||||
| N 28681-28752 | CDC_N3 | GGGAGCCTTGAATACACCAAAA | TGTAGCACGATTGCAGCATTG | ||||
| Human IPC | RNase P | CDC_RNAse P | AGATTTGGACCTGCGAGCG | GAGCGGCTGTCTCCACAAGT | 0.05~20 μM | [11] | |
IPC=Internal positive control