KCC2 is a direct target of miR-137. (A) Sequence alignment of miR-137 and the KCC2 3’-UTR, which contains a predicted conserved miR-137-biding site. The seed-recognizing site in the KCC2 3’-UTR is indicated in red, while the mutant KCC2 3’-UTR site is denoted in green. (B) KCC2 WT 3’-UTR-dependent expression of a Renilla luciferase was reduced, while mutation of the miR-137 binding site in the KCC2 3’-UTR did not affect the Renilla luciferase activity. (C) KCC2 mRNA expression levels were upregulated both in the hippocampus and in the prefrontal cortex of MIR137 cKO mice. (D) KCC2 protein expression levels were upregulated both in the hippocampus and in the prefrontal cortex of MIR137 cKO mice. Left panel, representative images of Western blot. Right panel, quantifications of Western blots with ImageJ. n=3 independent experiments; n.s non-significant, *p<0.05, **p<0.01.