KCC2 is upregulated upon the loss of miR-137 both in vitro and in vivo. (A) Representative images of immunostaining of hippocampal neurons at DIV-14) for KCC2 (green) and a mature neuronal marker MAP2 (red). Scale bars, 20 μm. (B) Quantitative analysis showing higher expression of KCC2 in MIR137 cKO hippocampal neurons at DIV-14. (C) Representative images of KCC2 immunostaining of MIR137 WT and cKO hippocampal sections. Scale bars, 50 μm. (D) Quantitative analysis demonstrating a higher KCC2 immunofluorescence intensity in MIR137 cKO hippocampus. (E) Representative images of KCC2 immunostaining of MIR137 WT and cKO prefrontal cortex sections. Scale bars, 50 μm. (F) Quantification showing an elevated KCC2 immunofluorescence signal in MIR137 cKO prefrontal cortex. n=4 mice per group; *p<0.05.