Primer sequences for RT-PCR
| Gene | Forward primer 5’-3’ | Reverse primer 5’-3’ | Product size |
|---|---|---|---|
| SIRT1 | GCAACAGCATCTTGCCTGAT | GTGCTACTGGTCTCACTT | 250 |
| SIRT2 | CTTCCTGGGCATGATGAT | ACCCTGACTGGGCATCTAT | 188 |
| SIRT3 | CAGCAACCTTCAGCAGTA | CCGTGCATGTAGCTGTTA | 278 |
| SIRT4 | TGACGGAGCTCCACGGATGC | ACACGCCGGTGCACAAAGTCA | 240 |
| SIRT5 | GTCATCACCCAGAACATTGA | ACGTGAGGTCGCAGCAAGCC | 251 |
| SIRT6 | TCCCAAGTGTAAGACGCAGT | GTTGCAGGTTGACAATGACC | 305 |
| SIRT7 | CCCCGGACCGCCATCTCA | CTCCAGGCCCAGTTCATTCAT | 453 |
| GAPDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGTA | 452 |