Changes in acetylated α-tubulin expression of the EpSCs during coculture with macrophages. (a) Detection of the expression of MAP-2 and acetylated α-tubulin (Ac α-tubulin) in the EpSCs after coculture with M0, M1 or M2 for 24 h. Significantly decreased Ac α-tubulin and increased MAP-2 were observed in the EpSCs cocultured with M2. (b) Detection of the temporal expression of Ac α-tubulin and α-tubulin in differently derived EpSCs when cocultured with M2 (0, 6, 12, 24 h) by western blotting assays. The Ac α-tubulin expression levels in the EpSCs derived from the WT mice gradually decreased from 24 to 48 h upon coculture with M2 and then returned to baseline at 72 h; in contrast, there were no apparent changes in the Ac α-tubulin expression in the EpSCs derived from the SIRT2-/- mice. Statistical analysis is shown in (c). Data represent three independent experiments. **p<0.01, ***p<0.001.