Summary of the effect of S274A mutation in diverse cell types
| Cell type | Cell name | NeuroD1 construct | Protein length (amino acid) | Localization | Transcriptional analysis | References | |
|---|---|---|---|---|---|---|---|
| Activity | Methods | ||||||
| Pancrease | MIN6 | Gal4-DBD-NeuroD1 | 156~355 | Cytosol>Nuclei | Low | CAT | [18] |
| βTC3 | Gal4-DBD-NeuroD1 | 156~355 | n.a. | Low | Luciferase | [17] | |
| INS-1 | Gal4-DBD-NeuroD1 | 156~355 | n.a. | Low | Luciferase | [17] | |
| INS-1 | Flag-NeuroD1 | 1~355 | n.a. | High | Luciferase | In this study | |
| Cervical cancer | HeLa | Flag-NeuroD1 | 1~355 | n.a. | High | Luciferase | In this study |
| Kidney | 293T | Flag-NeuroD1 | 1~355 | Cytosol | High | Luciferase | In this study |
| Neuroblastoma | P19 | Flag-NeuroD1 | 1~355 | n.a. | High | Luciferase | In this study |
| F11 | Flag-NeuroD1 | 1~355 | n.a. | High | Luciferase | In this study | |
DBD, DNA Binding Domain.