Fig. 1. RNA-sequencing analysis of isolated primary neurons from neurogenic differentiation 1 (NeuroD1) knockout (KO) mouse embryos at E18.5. (A) Scheme of the preparation of RNA for RNA-sequencing from primary neurons isolated from the NeuroD1 KO mouse forebrain. (B) Volcano plot analysis of differentially expressed genes (DEG). The number of up and downregulated genes are shown in the Table (Log2FC ≥0.5; p-value ≤0.05; for 3 animals per group). (C) The network of downregulated genes, identified by GeneMANIA software, evidencing seven key genes that are related to the function of NeuroD1 regarding axon development. Gray lines connect genes that are co-localized, and purple lines indicate co-expression. (D) TOP 10 rank of the Gene Ontology (GO) biological process and molecular functions. (E) Downregulation of the seven genes were validated using real-time quantitative PCR (RT-qPCR). (F) Protein to protein interaction analysis about NeuroD1 and axon development-related genes using STRING web software.
© Exp Neurobiol
