Fig. 2. Neurite outgrowth deficient in NeuroD1 knockout mouse cortical neurons. (A) Representative structure of microfluidic chambers with primary cortical neurons, isolated from the brain of NeuroD1 wild type (WT) and KO E16 embryos, plated in the cell body compartment (left). (B) Neurite lengths in the neurite compartment (right) separated by microgrooves (450 μm long, 10 μm wide) were measured at the 8th day in vitro (DIV). (C) Cortical neurons, at 8 DIV, were immunostained by microtubule-associated protein 2 (MAP2) (green) for dendrites and Hoechst (blue) for nuclei. Data are represented as means±S.E. from 27 WT and 21 KO neurons (***p<0.001, compared to the value of WT).
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