Detection methods and animal groups
| Detection methods | Animal groups |
|---|---|
| CTB tracing RGCs | Sham, Injury, AAV2-GFP, AAV2-Pim-1 |
| RITC tracing optic nerve | Sham, Injury, AAV2-GFP, AAV2-Pim-1 |
| Eosin-hematoxylin staining | Sham, Injury, AAV2-GFP, AAV2-Pim-1 |
| Immunohistochemical staining | Sham, Injury, AAV2-GFP, AAV2-Pim-1 |
| Immunofluorescence staining | AAV2-Pim-1 group without ONC |
| TdT-mediated dUTP Nick-End Labeling | Sham, Injury, AAV2-GFP, AAV2-Pim-1 |
| Quantitative real time polymerase chain reaction | Sham, AAV2-GFP, AAV2-Pim-1 groups without ONC |
| Sham, Injury, AAV2-GFP, AAV2-Pim-1 groups with ONC | |
| Western blotting | Sham, AAV2-GFP, AAV2-Pim-1 groups without ONC |
| Sham, Injury, AAV2-GFP, AAV2-Pim-1 groups with ONC | |
| Pupillary light reflex | Sham, Injury, AAV2-GFP, AAV2-Pim-1 |
| Flash visual evoked potentials | Sham, Injury, AAV2-GFP, AAV2-Pim-1 |