Primary antibodies and origin of the species for Western blotting
| Primary antibody | Cat | Species origin | Dilutions | Manufacturer |
|---|---|---|---|---|
| Stat3 | #9139 | Mouse | 1:1,000 | CST (Boston,USA) |
| p-Stat3 | #9134 | Rabbit | 1:1,000 | CST (USA) |
| Akt1 | #2938 | Rabbit | 1:1,000 | CST (USA) |
| p-Akt1 | ab81283 | Rabbit | 1:5,000 | Abcam (Cambridge,UK) |
| Akt2 | #3063 | Rabbit | 1:1,000 | CST (USA) |
| p-Akt2 | #8599 | Rabbit | 1:1,000 | CST (USA) |
| Erk1/2 | #4695 | Rabbit | 1:1,000 | CST (USA) |
| p-Erk1/2 | #4370 | Rabbit | 1:1,000 | CST (USA) |
| SOCS1 | #3950 | Rabbit | 1:1,000 | CST (USA) |
| SOCS3 | ab16030 | Rabbit | 1:1,000 | Abcam (UK) |
| Caspase 3 | #9665 | Rabbit | 1:1,000 | CST (USA) |
| Cleaved caspase 3 | #9664 | Rabbit | 1:1,000 | CST (USA) |
| Bad | ab32445 | Rabbit | 1:2,000 | Abcam (UK) |
| Bax | #14796 | Rabbit | 1:1,000 | CST (USA) |
| βIII-tubulin | ab18207 | Rabbit | 1:1,000 | Abcam (UK) |
| GAP-43 | ab75810 | Rabbit | 1:5,000 | Abcam (UK) |
| CXCR4 | ab124824 | Rabbit | 1:100 | Abcam (UK) |
| Met | sc-8057 | Mouse | 1:200 | Santa Cruz (Santa Cruz, Bolivia) |
| c-Myc | ab32072 | Rabbit | 1:5,000 | Abcam (UK) |
| 4E-BP1 | #9644 | Rabbit | 1:1,000 | CST (USA) |
| Pim-1 | sc-7857 | Goat | 1:200 | Biotech Well (Shanghai,China) |
| GAPDH | wb0197 | Mouse | 1:2,000 | CST (USA) |