Fig. 1. Timeline of animal experimental procedures. Part 1 (144 rats): Immediately after treatment, blood gas analysis was used to exclude signs of hypoxia. TUNEL, TEM, Western blot, real-time RT-PCR and IF in P7 rats 1 h, 8 h and 24 h post-treatment. Part 2 (174 rats): TUNEL and TEM assays 8 h post-treatment (time point: 0 h). Western blot, real-time RT-PCR and electrophysiological assays 1 month post-treatment. Water maze tests in 1- and 2-month-old rats. Drugs are distinguished by different colors (brown; yohimibine, blue; DEX, red; propofol). n=3 rats per group. Con, control; DEX, dexmedetomidine; IF, immunofluorescent staining; LTP, long term potentiation; TEM, transmission electronmicroscopy; TUNEL, Terminal deoxynucleotidyl transferase dUTP nick-end labeling; Y, yohimibine.
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