Fig. 1. The neural response properties of S1 L2/3 neurons evoked by static and dynamic stimuli applied to the right hind paw in lightly anesthetized mice. (A) (Top) A craniotomy was conducted over the primary somatosensory cortex (S1) corresponding to the right hind paw in the left hemisphere. In the lightly anesthetized head-fixed mice, static and dynamic stimuli were pseudorandomly applied with a custom-made vibrotactile stimulator, while S1 L2/3 neurons were recorded. (Bottom) Representative images of Ca2+ fluorescence of the S1 L2/3 neurons before and during static or dynamic stimuli. Scale bar, 20 μm. (B) Representative color-coded raster plots of responsive neurons in response to static or dynamic stimuli in the S1 L2/3. Each colored square box above the raster plots indicates when each static (purple) or dynamic (cyan) stimulus was given. Each stimulus was applied for 5 s. (C) The proportion of responsive neurons to static or dynamic stimuli (static, 18.42±7.28%; dynamic, 23.63±7.79%). (D) Response fidelity (%) of responsive neurons to static or dynamic stimuli (static, 45.63±3.27%; dynamic, 90.21±2.04%). (E) ΔF/F0 (%) of responsive neurons to static or dynamic stimuli (static, 237.78±21.24%; dynamic, 418.49±27.40%) in S1 L2/3 (n=126 cells from 5 mice; Two-tailed paired t-test, **p<0.01, ***p<0.001). All data are represented as mean±s.e.m.).
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