Durations of each sampling, RNA extraction and qRT-PCR procedure
| Pharyn/Manual | Pharyn/Kit | Saliva/Kit | Saliva/1-step Kit | |
|---|---|---|---|---|
| Self-pharyngeal swab collection | ~10 min | ~10 min | - | - |
| Self-saliva collection | - | - | ~10 sec | ~10 sec |
| RNA extraction | ~70 min | ~30 min | ~40 min | ~40 min |
| cDNA synthesis | 60 min | 60 min | 60 min | 5 min |
| qRT-PCR | 100 min | 100 min | 100 min | 65 min |
| Total time | ~4 hrs | ~3.3 hrs | ~3.3 hrs | ~2 hrs |