Human studies of NETs in stroke
| Author, year | Patients | Biomarkers | Method used in measurement of NETs | Results |
|---|---|---|---|---|
| Essig et al., 2020 [46] | 37 acute ischemic stroke (AIS) patients undergone mechanical thrombectomy | H3Cit, NE, MPO | Immunofluorescence | NETs found in more than 90% of thromboemboli. NETs were almost exclusively found within fibrin-rich areas |
| Zhou et al., 2020 [47] | 55 AIS patients undergone endovascular thrombectomy and 35 healthy controls | H3Cit, NE, MPO, cfDNA | Immunofluorescence | All NET-related markers found in plasma were higher in AIS compared with control group |
| Lim et al., 2020 [48] | 58 AIS patients and 25 healthy adults | Double-stranded DNA (dsDNA), DNA-histone complex | Immunofluorescence (for dsDNA) and ELISA (for DNA-histone complex) | Circulating levels of NETs was significantly higher in AIS patients at initial presentation compared with control group |
| Laridan et al., 2017 [49] | 68 AIS patients undergone thrombectomy | H3Cit | Immunostaining | NETs was observed in almost all thrombi |