Fig. 2. Astrocytic MAOB is responsible for MPTP-induced aberrant tonic inhibition. (A) Experimental schedule of tonic GABA recording from SNpc DA neurons of MPTP-treated MAOB-KO or WT littermate mice. (B) Representative traces of tonic GABA currents revealed by bath application of bicuculline (Bic, 50 mM) (C) Quantification of tonic GABA current (n=10 and 12 cells from 5 mice for each group). (D, E) Quantification of sIPSC frequency and amplitude. (F) Experimental schedule of tonic GABA recording from SNpc DA neurons of GFAP-MAOB mice treated with 75% dose of MPTP. (G) Representative traces of tonic GABA currents. (H) Quantification of tonic GABA current (n=5, 4, 11, and 10 cells for each group from 4, 3, 6, and 5 mice, respectively). (I, J) Quantification of sIPSC frequency and amplitude. For all figures, mean ± SEM; ns, non-significance; **p<0.01, ***p<0.001 assessed by Student’s t-test (C~E) or two-way ANOVA with Tukey (H~J).
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