Oligonucleotide primers and PCR conditions used for RT-PCR
| Gene | Forward | Reverse | Product size (bp) |
|---|---|---|---|
| NESTIN | AGGATGTGGAGGTAGTGAGA | TGGAGATCTCAGTGGCTCTT | 266 |
| BLBP | CGCTCCTGTCTCTAAAGAGGGG | TGGGCAAGTTGCTTGGAGTAAC | 594 |
| BMPRIB | CATGCTTTTGCGAAGTGCAG | CAGGCAACCCAGAGTCATCC | 197 |
| MASH1 | CCAACTACTCCAACGACTTG | GAAAGCACTAAAGATGCAGG | 194 |
| NCAM | GTCCTGCTCCTGGTGGTTGTG | CCTTCTCGGGCTCCGTCAGT | 264 |
| GFAP | CTGTTGCCAGAGATGGAGGTT | TCATCGCTCAGGAGGTCCTT | 382 |
| BMP2 | TCAAGCCAAACACAAACAGC | ACGTCTGAACAATGGCATGA | 200 |
| BMP4 | AAAGGGGCTTCCACCGTAT | CCGCTGTGAGTGCTTAG | 386 |
| hTERT | TGACACCTCACCTCACCCAC | CACTGTCTTCCGCAAGTTCAC | 95 |
| SOX2 | CCCCCGGCGGCAATAGCA | TCGGCGCCGGGGAGATACAT | 448 |
| GAPDH | AGCTGAACGGGAAGCTCACT | TGCTGTAGCCAAATTCGTTG | 297 |
| CD133 | TGGCAACAGCGATCAAGGAGAC | TCGGGGTGGCATGCCTGTCATA | 633 |
| EGFR | AACTGTGAGGTGGTCCTTGG | AGCTCCTTCAGTCCGGTTTT | 231 |
| MAP2 | GGGATTAGCAGTAACCCACG | AGGCCATCTGTCCAAAGTCA | 224 |
| NF200 | GCAGACATTGCCTACC | TCA CTC CTT CCG TCA CCC | 349 |
| TUJ1 | ACTTTATCTTCGGTCAGAGTG | CTCACGACATCCAGGACTGA | 97 |