Fig. 2. SVZ-neurogenic cellular composition of CN-derived cells and CN tissue. (a) Immunofluorescence staining of CN-differentiated cells at 1 week, showing localization of GFAP-delta+ (Green), and Tuj1+ cells (Red), and GFAP-delta+/Tuj1+(Green/Red) at one week after culture. (b) Immunofluorescence staining of neurogenic RGC cells (GFAP-delta+) showing morphology and arrangement indicative of newly divided cells. (c~f) Immunofluorescence staining of GFAP-delta+/Tuj1+ cells and Tuj1+ cells, showing asymmetrical divisions. (g~i) Tuj1+ cells localized and migrated along the fiber of GFAP-delta+/Tuj1+ (g, h) and GFAP-delta+ cells (i). (j) Example of Tuj1+ neuroblasts, which comprise the major population of CN. (k) Ki67 indicates mitotic and twin-like GFAP-delta+ cells. (l) The basal processes of GFAP-delta+/Tuj1+ cells were wrapped around Tuj1+ neuroblasts at 3 weeks after culture. (m) Immunofluorescence staining of CN tissue, showing localization of GFAP-delta+, GFAP-delta+/Tuj1+, and Tuj1+ labeled cells. (n) GFAP-delta+ cells from CN tissue, showing long processes similar to RGC. (o) TAP-like GFAP-delta+/Tuj1+ cells showing several processes proximal to Tuj1+cells. (p) Immunofluorescence staining of CN tissue showing clustered GFAP-delta+/Tuj1+ cells resembling daughter neuroblasts (Tuj1+). (q) Characteristics of three kinds of cells including GFAP-delta+ Type B, GFAP-delta+/Tuj1+ Type C, and Tuj1+ Type A cells. (r) Percentage of each type of cells (GFAP-delta+ NSC, GFAP-delta+/Tuj1+ TAP, Tuj1+ Neuroblast) in the population of CN: Type B (9.167±1.878 N=3), Type C (22.67±2.333 N=3), and Type A cells (67.47±2.784 N=3). (s) Representative FISH images carried out on these two tumors. Left: FISH images of 1p36 (red signal) as a target and of 1q24 (green signal) as a reference probe. Right: 19q13.3 (red signal) as a target and of 19p13 (green signal) as a reference. Two red and two green signals are evident, which implies no deletion of chromosome 1p and 19q. Scale bars, 50 µm (a) 100 µm (m) 10 µm (s). Three biological samples (N=3) were used for the experiments.
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