Fig. 5. Downregulation of TXNIP inhibited OGD/R-induced neurons apoptosis in vitro and ischemic brain injury in vivo. (A and B) Neurons were transfected with sh-TXNIP or sh-NC. The expression of TXNIP was detected by qRT-PCR (A) and western blot (B). (C, D) Neurons were transfected with sh-TXNIP or sh-NC, and then treated with OGD/R. (C) Cell viability was evaluated by MTT assay. (D) Apoptotic rate was detected by flow cytometry. *p<0.05, **p<0.01. (E-H) sh-TXNIP or sh-NC was injected into the cerebral cortex of rats, and rats were then treated with MCAO/R. The expression of TXNIP in ischemic brain was evaluated by qRT-PCR (E) and western blot (F). (G) Neurobehavioral scores. (H) The representative images of brains tissues and infarct volume. *p<0.05, **p<0.01.
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