Fig. 6. Upregulation of miR-20b attenuated OGD/R-induced neurons apoptosis in vitro and ischemic brain injury through targeting TXNIP. (A-C) Neurons were transfected with miR-20b mimics, miR-20b inhibitor, or co-transfected with miR-20b mimics and sh-TXNIP, or miR-20b inhibitor and sh-TXNIP. Then cells were treated with OGD/R. (A) The expression of TXNIP was evaluated by western blot. (B) Cell viability was detected by MTT assay. (C) Apoptotic rate was measured by flow cytometry. *p<0.05, **p<0.01. (D) Rats were cortical injected with miR-20b mimics, miR-20b inhibitor, or co-injection with miR-20b mimics and sh-TXNIP, or miR-20b inhibitor and sh-TXNIP. Then rats were treated with MCAO/R. The representative images of brains tissues and infarct volume. *p<0.05, **p<0.01.
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