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Fig. 1. Cutting-edge tools for measuring spot distance in 3D SIM images (Lattice SIM and Imaris XT distance transformation). (A) Schematic principle of diagram of lattice SIM. (B) High magnification image taken by lattice SIM and spot identification and surface rendering 3D image processed by Imaris9.0. (C) Validation of antibody against Best1 in CA1 of WT and Best1 KO mice. The images were taken by Axioscan 7 (Zeiss, Germany).
Exp Neurobiol 2021;30:213~221
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