The primer sequences for PCR reaction
| Target | Primer sequence | PCR product |
|---|---|---|
| Forward | 5’-AAA GTC GCT CTG AGT TGT TAT-3’ | WT: 600 bp (F+R1) Knock-in: 325 bp (F+R2) |
| Reverse 1 | 5’-GGA GCG GGA GAA ATG GAT ATG-3’ | |
| Reverse 2 | 5’-GCG AAG AGT TTG TCC TCA ACC-3’ | |
| Forward | 5’-GCG AAG AGT TTG TCC TCA ACC-3’ | 750 bp |
| Reverse | 5’-ATG GCG GAC TTG AAG AAG TCG TG-3’ | |
| Forward | 5’-GCA TTA CCG GTC GAT GCA ACG AGT GAT GAG-3’ | 404 bp |
| Reverse | 5’-GAG TAG ACG AAC CTG GTC GAA ATC AGT GCG-3’ |