Fig. 3. miR-365 overexpression inhibited the stemness and TMZ resistance of glioma cells. (A) The expression level of miR-365 was successfully up-regulated in U87 and U251 cells after transfected with miR-365 mimics, as detected by RT-qPCR. (B) miR-365 overexpression inhibited glioma cell proliferation, as indicated by CCK8. (C) MiR-365 overexpression inhibited glioma cell migration indicated by wound healing assay. (D) miR-365 overexpression inhibited glioma cell invasion, as shown by transwell assay. (E) The rate of CD133+ U87 and U251 cells was suppressed after miR-365 overexpression, as demonstrated by flow cytometry assay. (F) The protein level of ELF4 and stemness factors (SOX2, Oct4, Nanog) were inhibited by miR-365 overexpression in glioma cells, as indicated by western blot. GAPDH was used for normalization. (G) Overexpression of miR-365 reduced glioma cell viability and sensitized cells to TMZ, as detected by CCK8 assay. Data are presented as Mean±SD of three independent experiments. *p<0.05, **p<0.01, ***p<0.001.
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